BACKGROUND
A team of researchers from the University of Calgary have developed five homozygous knock-out cancer cell lines developed on the background of the THP-1 leukemic monocyte. These cell lines may be used as a tool in blood cancer and immunological research, and for screening potential therapeutic candidates. The genotypes are summarized below.
GENOTYPES
- Target gene: NLRP3 (NOD-like receptor containing pyrin 3)
- Cell line: THP-1 Monocyte
- Organism: Human
- Gene editing method: CRISPR/CAS9
- Parental cell line: ATCC TIB-202
- Target gene: CASPR 4 (Caspase 4)
- Cell line: THP-1 Monocyte
- Organism: Human
- Gene editing method: CRISPR/CAS9
- Parental cell line: ATCC TIB-202
- Target gene: GSDMD (Gasdermin-D)
- Cell line: THP-1 Monocyte
- Organism: Human
- Gene editing method: CRISPR/CAS9
- Parental cell line: ATCC TIB-202
- Target gene: ERN1 (Serine/ threonine protein kinase/endonuclease IRE, ERN1)
- Cell line: THP-1 Monocyte
- Organism: Human
- Gene editing method: CRISPR/CAS9
- Parental cell line: ATCC TIB-202
- Target gene: AIM2 (Absent in Melanoma 2)
- Cell line: THP-1 Monocyte
- Organism: Human
- Gene editing method: CRISPR/CAS9
- Parental cell line: ATCC TIB-202
AREAS OF APPLICATION
- Blood cancer research
- Immunology research
- Drug screening
PUBLICATIONS AND RESOURCES
- Journal publications
- Platnich JM et al. (2018). Shiga toxin/lipopolysaccharide activates Caspase-4 and Gasdermin D to trigger mitochondrial reactive oxygen species upstream of the NLRP3 inflammasome. Cell Rep 25(6): 1525-1536.
- Lau A et al. (2018). Renal immune surveillance and dipeptidase-1 contribute to contrast-induced kidney injury. J Clin Invest 128(7): 2894-2913.
- Quach J et al. (2018). Entamoeba histolytica-induced IL-1β secretion is dependent on caspase-4 and gasdermin D. Mucosal Immunology 12: 323-339.
- Hudson GM et al. (2019). Pregnane X receptor activation triggers rapid ATP release in primed macrophages that mediates NLRP3 inflammasome activation. J Pharmacol Exp Ther 370(1): 44-53.
- Researcher Profile: Dr. Justin MacDonald