Researchers at the University of Calgary have created a point-of-care (POC) biosensor platform for any small molecule or biological samples. This biosensor relies on cognate enzyme complementation and produces glucose as a final product. The resultant glucose can be easily measured using common or custom-build glucometers. This new method provides a way to detect a wide range of analytes in various biological samples, including blood and colostrum, with a high degree of specificity and sensitivity.
AREAS OF APPLICATION
Verified in clinical samples:
- Small molecule detection – bovine serum calcium test for hypocalcaemia diagnosis1.
- Antibody detection – sera antibody test against bovine leukemia virus (BLV)2,3.
- Immunoglobulin detection:
- Serum IgG concentration test in various animal species4.
- Colostrum IgG concentration test5.
- Genetic targets, after amplicons by PCR or isothermal amplification (LAMP)6.
- Protein aggregation detection3.
- High sensitivity and specificity demonstrated on various clinical samples.
- Portable, inexpensive, and simple to use – direct use on biological samples, no purification or processing needed.
- Fast – test results can be available within 10 minutes.
- The inventor has access to large animal resources for clinical trials.
PUBLICATIONS AND RESOURCES
- Patent publication: US10900960B2
- Journal publications:
- Fu Y et al. (2022). Development of a blood calcium test for hypocalcemia diagnosis in dairy cows. Res Vet Sci 147: 60-67.
- Mukherjee S & De Buck J. (2021). Autotransporter-based surface expression and complementation of split TreA fragments utilized for the detection of antibodies against bovine leukemia virus. J Immunol Methods 495: 113084.
- Drikic M & De Buck J. (2018). Split trehalase as a versatile for a wide range of biological analytes. Biotechnol Bioeng 115(5): 1128-1136.
- Drikic M et al. (2019). Detecting total immunoglobulins in diverse animal species with a novel split enzymatic assay. BMC Vet Res 15(1): 374.
- Drikic M et al. (2018). Determining the IgG concentrations in bovine colostrum and calf sera with a novel enzymatic assay. Journal of Animal Science and Biotechnology 9(69).
- Zhuohan M. (2022). Development of a gram-type specific method for detection of bovine mastitis pathogens by combining loop-mediated isothermal amplification (LAMP) and split trehalase technologies. University of Calgary.
- Researcher profile: Jeroen De Buck
- Lab website: De Buck Lab